FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT
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چکیده
Mesoderm induction begins during gastrulation. Recent evidence from several vertebrate species indicates mesoderm induction continues after gastrulation in neuromesodermal progenitor cells (NMPs) within the posterior-most embryonic structure called the tailbud. It is unclear to what extent the molecular mechanisms of mesoderm induction are conserved between gastrula and postgastrula stages of development. Fibroblast growth factor (FGF) signaling is required for mesoderm induction during gastrulation through positive transcriptional regulation of the t-box transcription factor brachyury (ntla in zebrafish). We find that FGF is continuously required for paraxial mesoderm (PM) induction in post-gastrula NMPs, but has the opposite effect on brachyury expression. FGF signaling represses brachyury and the NMP marker sox2 through regulation of tbx16 and msgn1, thereby committing cells to a PM fate. FGF mediated PM induction in NMPs functions in tight coordination with canonical Wnt signaling during the epithelial to mesenchymal transition from NMP to mesodermal progenitor. Wnt signaling initiates the EMT, while FGF signaling terminates this event. Our results indicate that germ layer induction in the tailbud is not a simple continuation of gastrulation events. D ev el o pm en t • A dv an ce a rt ic le http://dev.biologists.org/lookup/doi/10.1242/dev.143578 Access the most recent version at Development Advance Online Articles. First posted online on 27 February 2017 as 10.1242/dev.143578
منابع مشابه
FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step epithelial to mesenchymal transition.
Mesoderm induction begins during gastrulation. Recent evidence from several vertebrate species indicates that mesoderm induction continues after gastrulation in neuromesodermal progenitors (NMPs) within the posteriormost embryonic structure, the tailbud. It is unclear to what extent the molecular mechanisms of mesoderm induction are conserved between gastrula and post-gastrula stages of develop...
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Vertebrate body axis formation depends on a population of bipotential neuromesodermal cells along the posterior wall of the tailbud that make a germ layer decision after gastrulation to form spinal cord and mesoderm. Despite exhibiting germ layer plasticity, these cells never give rise to midline tissues of the notochord, floor plate and dorsal endoderm, raising the question of whether midline ...
متن کاملFgf and canonical Wnt signaling form a network with T-box family transcription factors to govern cell fates within the tailbud
INTRODUCTION Vertebrate axis elongation intertwines cell migration, cell differentiation and tissue patterning within a structure called the tailbud. The tailbud is the posterior end of the growing trunk and tail and contains both neural and mesodermal anlagen (Griffith et al., 1992; Holmdahl, 1925; Wilson et al., 2009). Genetic and fatemapping studies demonstrate the existence of a population ...
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